Journal: bioRxiv
Article Title: STRAIN-SPECIFIC VIRULENCE SIGNATURES OF CAMPYLOBACTER JEJUNI ASSOCIATED WITH WATERY VERSUS BLOODY DIARRHEA IN THE NEONATAL PIGLET MODEL
doi: 10.1101/2025.04.25.650680
Figure Lengend Snippet: Adherence is expressed as the percentage of bacteria which had either adhered to or invaded cultured intestinal epithelial cells following a 1-hour incubation, compared to the inoculum. (A) Adherence of each C. jejuni strain to INT407 cells. Bars labeled with different letters indicate statistically significant differences (p < 0.05) based on Dunn’s post hoc test following a Kruskal-Wallis analysis (n = 3). Shared letters denote groups that are not significantly different from each other. (B) Adherence to INT407 cell by C. jejuni strains grouped by diarrheal manifestation. There was a significant difference between watery and bloody/inflammatory diarrhea associated strains and their attachment to INT407 cells (*, p = 0.028). Average percent attachment was calculated from three biologically independent assays with two technical replicates (n = 15). (C) Adherence of each C. jejuni strain to Caco-2 BBE cells (n = 3). (D) Adherence to Caco-2 BBE cells by C. jejuni strains grouped by diarrheal manifestation. There was a significant difference between watery and bloody/inflammatory diarrhea associated strains and their attachment to Caco-2 BBE cells (*, p = 0.023). Average percent attachment was calculated from three biologically independent assays with two technical replicates (n = 15).
Article Snippet: Attachment was assessed using three epithelial cell lines: non-polarized INT407 (ATCC CCL-6), and polarized Caco-2 BBE (ATCC HTB-37) and T84 (ATCC CCL-248) cells.
Techniques: Bacteria, Cell Culture, Incubation, Labeling